Reverse PCR

Identification of native plasmid in a Rhodopseudomonas palustris strain by reverse PCR

利用反向PCR鉴定沼泽红假单胞菌中野生质粒

Cloning haemolysin gene of Vibrio alginolyticus through reverse PCR and expression of the gene in prokaryotic cell

溶藻弧菌溶血素基因反向PCR克隆及其原核表达

The result proved an improved reverse PCR method applied to T-DNA flanking sequence analysis of the transgenic birch was feasibility and rationality .

证明改良的反向PCR方法运用到转基因白桦T-DNA旁侧序列的可行性和合理性。

For special aims , multiple PCR , reverse PCR , quantitative PCR , in situ PCR , as well as capillary PCR , were developed .

而适合不同目的的PCR技术也得到了充分的发展，如多重PCR、反转录PCR、定量PCR、原位PCR、PCR突变、毛细管PCR技术等等。

Porcine CD86 was amplified by RT-PCR from porcine alveolar macrophages and cloned . Following sequencing , competitive depletion clones for the cDNA molecules was generated by reverse PCR .

用RT-PCR技术从猪肺泡巨噬细胞中扩增和克隆了猪CD86基因部分片段，经测序鉴定后，用反向PCR技术构建了CD86的缺失竞争cDNA分子。

Detection of Hepatitis A Virus by Two Reverse Transcription PCR Approaches

两种反转录聚合酶链反应检测甲型肝炎病毒

Application of in situ reverse transcription PCR techniques

原位反转录PCR技术的应用

Application of reverse transcription PCR in the detection of respiratory syncytial virus infection

反转录-聚合酶链反应在呼吸道合胞病毒感染检测的应用

Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR

实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型

CDNA-AFLP differential patterns for 5 selected genes were confirmed via real-time reverse transcription PCR analysis .

用荧光实时定量PCR分析法证实了cDNA-AFLP基因表达结果。

The gene expression of a-actin mRNA were determined by using the quantitative reverse transcription PCR technology .

利用半定量反转录聚合酶链式反应（QRT-PCR）法测定骨骼肌α-actin基因表达。

Identification of Viruses Causing Respiratory Tract Infection by Single-tube Multiplex Reverse Transcription PCR

应用多重反转录PCR技术检测病毒性呼吸道感染

A reverse transcription PCR system was established for the detection of Noroviruses in shellfish .

本文建立了贝类产品中诺沃克病毒检测的普通RT-PCR方法。

A coupled one-step reverse transcription PCR procedure for cloning the human augmenter of liver regeneration gene

RT-PCR一步法克隆人肝再生增强因子基因

Hantavirus RNA detection in clinical sera of hemorrhagic fever with renal syndrome using reverse transcription-nested PCR

应用反转录-套式PCR方法检测肾综合征出血热病人血清中的汉坦病毒特异性RNA

A Reverse Transcription PCR that Distinguishes the Isolates of Low Virulent Strains From Virulent Strains of Newcastle Disease Virus

鸡新城疫病毒强弱毒株RT-PCR鉴别诊断方法

An improved differential display reverse transcript PCR ( DDRT-PCR ) approach was used to identify novel genes treated by iron-deficiency from rice cells .

利用改进的差异显示PCR（DDRT-PCR）技术从水稻细胞中分离与缺铁胁迫相关的新基因。

Real-time fluorescence quantitative reverse transcription PCR in determination of guanylyl cyclase-C gene expression in peripheral blood of patients with colorectal cancer and its clinical significance

实时荧光定量PCR检测大肠癌患者外周血鸟苷酰环化酶C基因及其临床意义

Reverse transcription PCR and in situ hybridization were used to detect the expression changes of CaBP mRNA in the brain of PD and control mice .

用反转录PCR及原位分子杂交方法检测PD鼠和正常鼠脑内CaBPmRNA的表达变化。

The silencing effects of TIMP-1 on the mRNA and protein levels were examined by reverse transcription PCR ( RTPCR ) and Western blot analysis respectively .

采用RT-PCR和Western印迹测定宿主细胞TIMP-1在基因和蛋白水平的沉默效果。

Recombinant human EGF was used to stimulate HepG_2 cells and semi-quantitative reverse transcription PCR was adopted to detect the expression of VEGF in HepG_2 cells .

离体实验中，用重组人EGF刺激人肝癌细胞系HepG2，采用半定量逆转录PCR检测VEGF的表达情况。

Methods The interested segment was obtained by reverse transcription PCR with the designed specific primers , and inserted into pMD-18T vector by T / A match .

方法设计特异引物，用RT-PCR方法从乳腺癌组织扩增获得目的片段，利用T/A克隆将PCR产物插入pMD-18T载体；

The semi - quantitative reverse transcriptive PCR was performed to detect the expression of TGF - β 1 , CTGF and FN mRNA .

RT-PCR半定量分析HK2细胞中TGF-β1、CTGF和FnmRNA的表达水平；

Objective To investigate the differentially expressed gene profile between high rate and low rate metastatic large cell lung cancer cell lines via differential display reverse transcript PCR ( DDRT PCR ) .

目的通过研究人高、低转移肺大细胞癌细胞系基因差异表达谱，克隆与肿瘤转移相关的基因。

Roles of the EnvZ / OmpR two-component system in response to various stresses were investigated with mutational analysis , quantitative reverse transcriptase PCR ( qRT-PCR ) .

同时通过突变分析、定量逆转录聚合酶链反应（qRT-PCR）研究了EnvZ/OmpR二元系统在应对渗透压力中的作用。

The method of reverse transcription PCR ( RT-PCR ) is more sensitive and reliable than that of enzyme-linked immunosorbent assay on nitrocellulose membranes ( NCM-ELISA ) .

对于甘薯病毒病的检测，反转录PCR法（RT-PCR）比硝化纤维素膜酶联免疫吸附检测法（NCM-ELISA）更灵敏和可靠。

Extraction of total RNA from culture clones which were expanded , and then detecting the target gene was integrated into the genome of HeLa cells through reverse transcriptase PCR method ( RT-PCR ) .

对单克隆进行扩大培养，提取其总RNA，逆转录酶PCR法（Reversetranscriptase-PCR，RT-PCR）检测到目的基因已整合到HeLa细胞基因组中，说明重组质粒在细胞中稳定表达。

Methods Total RNA in the metastatic lymph nodes from patients with colorectal cancer was extracted routinely , and reverse transcriptase PCR employed to amplify the heavy chain Fd and light chain κ cDNA .

方法取大肠癌病人转移淋巴结，提取淋巴结总RNA，逆转录PCR扩增重链Fd和K轻链cDNA。

Methods : By using the reverse transcriptional PCR and in situ hybridization , the expression changes of FHF 2 mRNA were studied in DRG of normal rats and rats after transection of the peripheral nerve .

方法：应用逆转录PCR和原位杂交技术，研究正常大鼠和外周神经切断后大鼠DRG内FHF2mRNA的表达变化。

Methods Techniques of cell culture in vitro and RT PCR ( reverse transcription PCR ) were used , gene expressions of p53 and fas in 4 tumor cell lines were measured after treated with ethanol extractive and water extractive .

方法采用肿瘤细胞体外培养技术及RT-PCR技术，观察阿魏菇不同剂量的水提物及醇提物对体外培养的四种肿瘤细胞p53及fas基因表达的影响。